Potent monoclonal antibodies against Clostridium difficile toxin A elicited by DNA immunization

 Abstract

Recent studies have demonstrated that DNA immunization is effective in eliciting antigen-specific antibody responses against a wide range of infectious disease targets. The polyclonal antibodies elicited by DNA vaccination exhibit high sensitivity to conformational epitopes and high avidity. However, there have been limited reports in literature on the production of monoclonal antibodies (mAb) by DNA immunization. Here, by using Clostridium difficile (C. diff) toxin A as a model antigen, we demonstrated that DNA immunization was effective in producing a panel of mAb that are protective against toxin A challenge and can also be used as sensitive reagents to detect toxin A from various testing samples. The immunoglobulin (Ig) gene usage for such mAb was also investigated. Further studies should be conducted to fully establish DNA immunization as a unique platform to produce mAb in various hosts.

Full Text Options
Article
Metrics
 Share
 Full Text
 Info
Pages
2157 - 2164
doi
10.4161/hv.25656
Type
Research Paper
Group
DNA Vaccines
 Metrics
 Cite This Article
 Permissions
 Permissions
 Reprints
Potent monoclonal antibodies against Clostridium difficile toxin A elicited by DNA immunization