Heterochromatin protein 1a is required for an open chromatin structure

 Abstract

The Drosophila melanogaster fourth chromosome contains interspersed domains of active and repressive chromatin. We investigated a stock harboring a silenced transgene inserted into Dyrk3 and near Caps—two expressed genes on chromosome four. In an HP1a-deficient background, transgene expression was activated, while, paradoxically, expression of Dyrk3 and Caps was reduced. We found that the promoters of Dyrk3 and Caps contained DNase I hypersensitive sites, but also possessed methylated histone H3 and HP1a, marks of repressive chromatin. In HP1a-deficient flies, the Dyrk3 and Caps promoters displayed diminished accessibility to nuclease digestion, revealing a surprising role for HP1a in opening chromatin.

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Pages
95 - 99
doi
10.4161/trns.2.2.14687
Type
Research Paper
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Heterochromatin protein 1a is required for an open chromatin structure