Proinflammatory fecal mRNA and childhood bacterial enteric infections

 Abstract

Introduction: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. Methods: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp., or Shigella sonnei and 26 controls, and compared the proportions of IL-1β, IL-8, osteoprotegerin, and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8, and osteoprotegerin by enzyme immunoassays in cognate specimens. Results: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8, and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. Discussion: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA.

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Pages
209 - 212
doi
10.4161/gmic.1.4.13004
Type
Brief Report
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Proinflammatory fecal mRNA and childhood bacterial enteric infections