Accurate sodium bisulfite sequencing in plants

 Abstract

DNA cytosine methylation is a conserved epigenetic modification frequently correlating with transcriptional silencing in a wide variety of eukaryotic organisms. Sodium bisulfite treatment of DNA converts unmethylated cytosine to uracil, while 5-methylated cytosine is protected. We describe techniques that ensure reliable sequencing data following sodium bisulfite conversion and to avoid common pitfalls such as amplification of unconverted DNA and inclusion of sibling clones.

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Pages
141 - 143
doi
10.4161/epi.5.1.10560
Type
Methods and Technical Advances
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Accurate sodium bisulfite sequencing in plants