Evidence That the Yeast Spindle Assembly Checkpoint Has a Target Other Than the Anaphase Promoting Complex

 Abstract

The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.

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Pages
1555 - 1557
doi
10.4161/cc.4.11.2144
Type
Special Report
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Evidence That the Yeast Spindle Assembly Checkpoint Has a Target Other Than the Anaphase Promoting Complex