Tap42-associated protein phosphatase type 2A negatively regulates induction of autophagy

 Abstract

Autophagy is a highly conserved degradative process in eukaryotic cells. This process plays an integral role in cellular physiology, and the levels of autophagy must be precisely controlled to prevent cellular dysfunction. The rapamycin-sensitive Tor kinase complex 1 (TORC1) has a major role in regulating the induction of autophagy; however, the regulatory mechanisms are not fully understood. Here, we find that Tap42 and protein phosphatase type 2A (PP2A) are involved in the regulation of autophagy in yeast. Temperature-sensitive mutant alleles of TAP42 revealed that autophagy was induced without inactivation of TORC1. Absence of the Tap42-interacting protein Tip41 abolished autophagy induction in the tap42 mutants, whereas overexpression of Tip41 activated autophagy. Furthermore, inactivation of PP2A stimulated autophagy and overexpression of a catalytic subunit of PP2A blocked rapamycin-induced autophagy. Our data support a model in which autophagy is negatively regulated by the Tap42-PP2A pathway.

Full Text Options
Article
Metrics
 Share
 Info
Pages
616 - 624
doi
10.4161/auto.5.5.8091
Type
Research Paper
 Metrics
 Cite This Article
 Permissions
 Permissions
 Reprints
Tap42-associated protein phosphatase type 2A negatively regulates induction of autophagy