Yeast vacuole fusion: A model system for eukaryotic endomembrane dynamics

 Abstract

Vesicular transport in eukaryotic cells is concluded with the consumption of the vesicle at the target membrane. This fusion process relies on Rabs, tethers and SNAREs. Powerful in vitro fusion systems using isolated organelles were crucial to obtain insights into the underlying mechanism of membrane fusion—from the initiation of fusion to lipid bilayer mixing. Among these systems, yeast vacuoles turned out to be particularly useful as they can be manipulated biochemically and genetically, and can be analyzed by fluorescence microscopy techniques, and studies relying on this organelle have revealed insights into the connection of vacuole fusion to endomembrane biogenesis. A number of fusion factors were identified and characterized over the last several years, and placed into the fusion cascade. Within this review, we will present and discuss the current state of our knowledge on vacuole fusion.

Full Text Options
Article
Metrics
 Share
 Full Text
 Info
Pages
5 - 19
Type
Review
 Metrics
 Cite This Article
 Permissions
 Permissions
 Reprints
Yeast vacuole fusion: A model system for eukaryotic endomembrane dynamics