A genomic screen for yeast mutants defective in mitophagy

 Abstract

Mitochondria autophagy (mitophagy) is the process of selective degradation of mitochondria that has an important role in mitochondrial quality control. To gain insight into the molecular mechanism of mitophagy, we screened a yeast knockout library for strains that are defective in mitophagy. We found 32 strains that showed a complete or partial block of mitophagy. One of the genes identified, YLR356W, is required for mitophagy, but not for macroautophagy or other types of selective autophagy. The deletion of YLR356W partially inhibits mitophagy during starvation, whereas there is almost complete inhibition at post-log phase. Accordingly, we hypothesize that Ylr356w is required to detect or present aged or dysfunctional mitochondria when cells reach the post-log phase.

 Related Article:

T Kanki, K Wang, M Baba, CR Bartholomew, MA Lynch-Day, Z Du, J Geng, K Mao, Z Yang, WL Yen, DJ Klionsky. A genomic screen for yeast mutants defective in selective mitochondria autophagy. Mol Biol Cell 2009; 20: 4730- 8.
PMID: 19793921 DOI: 10.1091/mbc.E09-03-0

Full Text Options
Article
Metrics
 Share
 Full Text
 Info
Pages
278 - 280
doi
10.4161/auto.6.2.10901
Type
Autophagic Punctum
 Metrics
 Cite This Article
 Permissions
 Permissions
 Reprints
A genomic screen for yeast mutants defective in mitophagy