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Autophagy Reagent ForumAnti-LAMP-2November 21st, 2008Company: Invitrogen Catalog #: 51-2200 Comments: Rabbit antibody that recognizes the cytosolic tail of rat and mouse LAMP-2A for the analysis of chaperone-mediated autophagy. Posted by: Daniel J. Klionsky Institution: University of Michigan anti-hsc70November 21st, 2008Company: Abcam Catalog #: ab2788 Comments: This mouse monoclonal antibody 13D3 recognizes hsc70, the chaperone needed for chaperone-mediated autophagy. Posted by: Daniel J. Klionsky Institution: University of Michigan MitoTracker Green FMNovember 21st, 2008Company: Molecular Probes/Invitrogen Catalog #: M-7514 Comments: This is a membrane potential-independent mitochondrial dye that can be used to follow mitophagy. This dye is sensitive to fixation.
Posted by: Daniel J. Klionsky Institution: University of Michigan MitoTracker Red CMXRosNovember 21st, 2008Company: Molecular Probes/Invitrogen Catalog #: M-7512 Comments: This is a membrane potential-dependent mitochondrial dye that can be used to follow mitophagy.
Posted by: Daniel J. Klionsky Institution: University of Michigan anti-LC3 (human)November 21st, 2008Company: Novus Biologicals Catalog #: NB100-2331 Comments: This is a rabbit polyclonal antibody raised to a peptide corresponding to amino acids 50-150 of human LC3. My lab has used this antibody with extracts from human cell lines and obtained excellent results with western blot. Posted by: Daniel J. Klionsky Institution: University of Michigan anti-phospho-mTOR Ser2448November 21st, 2008Company: Cell Signaling Catalog #: 2976 Comments: This rabbit monoclonal 49F9 can be used for immunohistochemistry. Posted by: Daniel J. Klionsky Institution: University of Michigan Anti-phospho-S6K Ser235/236November 21st, 2008Company: Cell Signaling Catalog #: 4857 Comments: This rabbit monoclonal 91B2 can be used for immunohistochemistry. Posted by: Daniel J. Klionsky Institution: University of Michigan anti-mTOR (FRAP)November 21st, 2008Company: Santa Cruz Biotechnology Catalog #: sc-1549 Comments: Antibody N-19 is a goat polyclonal antibody that can be used to immunoprecipitate mTOR. Posted by: Daniel J. Klionsky Institution: University of Michigan Anti-phospho-4E-BP1 Thr70November 21st, 2008Company: Cell Signaling Catalog #: 9455 Comments: This antibody can be used to examine the phosphorylation of 4E-BP1, a substrate of mTORC1. Posted by: Daniel J. Klionsky Institution: University of Michigan Anti-phospho-4E-BP1 Ser65November 21st, 2008Company: Cell Signaling Catalog #: 9451 Comments: This antibody can be used to examine the phosphorylation of 4E-BP1, a substrate of mTORC1. Posted by: Daniel J. Klionsky Institution: University of Michigan |