• A new tool in <em>C. elegans</em> reveals changes in secretory protein metabolism in <em>ire-1</em>-deficient animals
  • Worm variation made accessible: Take your shopping cart to store, link, and investigate!
  • Nematode endogenous small RNA pathways
  • From genes to circuits and behaviors: Neuropeptides expand the coding potential of the nervous system

January 1, 2014

 A new tool in C. elegans reveals changes in secretory protein metabolism in ire-1-deficient animals

Modi Safra and Sivan Henis-Korenblit

We recently showed that the ire-1/xbp-1 arm of the UPR plays a crucial role in maintaining basic endoplasmic reticulum (ER) functions required for the metabolism of secreted proteins even during unstressed growth conditions. During these studies we realized that although C. elegans is a powerful system to study the genetics of many cellular processes; it lacks effective tools for tracking the metabolism of secreted proteins at the cell and organism levels. Here, we outline how genetic manipulations and expression analysis of a DAF-28::GFP translational fusion transgene can be combined to infer different steps in the life cycle of secretory proteins. We demonstrate how we have used this tool to reveal folding defects, clearance defects, and secretion defects in ire-1 and xbp-1 mutants. We believe that further studies using this tool will deepen the understanding of secretory protein metabolism.


January 1, 2014

 Worm variation made accessible: Take your shopping cart to store, link, and investigate!

L Basten Snoek, K Joeri van der Velde, Yang Li, Ritsert C Jansen, Morris A Swertz and Jan E Kammenga


January 1, 2014

 Nematode endogenous small RNA pathways

Suzanne W Hoogstrate, Rita JM Volkers, Mark G Sterken, Jan E Kammenga and L Basten Snoek


January 1, 2014

 From genes to circuits and behaviors: Neuropeptides expand the coding potential of the nervous system

Sarah G Leinwand and Sreekanth H Chalasani


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