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Research Paper
Development, Characterization and Immunogenicity of a Multi-Stage, Multivalent Plasmodium falciparum Vaccine Antigen (FALVAC-1A) Expressed in Escherichia coli
Zhiyong Zhou, Charles W. Todd, Robert M. Wohlhueter, April Price, Lihua Xiao, Paul Schnake, Phillip Cullison, Amy M. Martin, Ira F. Goldman, Patricia De La Vega, Venkatachalam Udhayakumar and Altaf A. Lal
volume 2 | issue 1
january/february 2006Pages: 14 - 23
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A synthetic multistage, multi-epitope Plasmodium falciparum malaria antigen (FALVAC-1A) was designed and evaluated in silico, and then the gene was constructed and expressed in Escherichia coli. The FALVAC-1A protein was purified by inclusion body isolation, followed by affinity and ion exchange chromatography. Although FALVAC-1A was a synthetic antigen, it folded to a specific, but as yet incompletely defined, molecular conformation that was stable and comparable from lot to lot. When formulated with 4 different adjuvants, FALVAC-1A was highly immunogenic in rabbits, inducing not only ELISA reactivity to the cognate antigen and most of its component epitopes, but also in vitro activity against P. falciparum parasites as demonstrated by inhibition of sporozoite invasion, antibody dependent cellular inhibition and the immunofluorescence assay.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.