Comparison of stimulating effect on subpopulations of lymphocytes in human peripheral blood by methionine enkephalin with IL-2 and IFN-γ
Volume 8, Issue 8
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Pages 1082 - 1089http://dx.doi.org/10.4161/hv.20759
: Interferon-γ, Interleukin-2, human peripheral blood, lymphocyte subpopulations, methionine enkephalin
Authors: Hui Hua, Changlong Lu, Weiwei Li, Jingjuan Meng, Danan Wang, Nicolas P. Plotnikoff, Enhua Wang and Fengping Shan View affiliations
The aim of this study was to investigate the effects of mechanisms of methionine enkephalin (MENK) on lymphocytes in human peripheral blood. We detected CD4+T cells, CD8+T cells, CD4+CD25+ regulatory T cells (Treg), dendritic cells (DCs), natural killer cells (NK), NKT cells and γδT cells before and after treatment with 10−12M MENK, in cell culture by FCM and RT-PCR. Our findings show that MENK stimulating expansion of lymphocyte subpopulationns by inhibiting CD4+CD25+ regulatory T cells (Treg), which is unique discovery of our study. We may use MENK as a drug to treat cancer patients, whose immune systems are damaged by chemotherapy or radiotherapy.
Received: February 22, 2012; Accepted: May 15, 2012; Published Online: August 1, 2012
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