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Research Paper
Immunogenicity of the Hookworm Na-ASP-2 Vaccine Candidate: Characterization of Humoral and Cellular Responses after Vaccination in the Sprague Dawley Rat
Ricardo T. Fujiwara, Jeffrey Bethony, Lilian L. Bueno, Yan Wang, So Yeong Ahn, Andre Samuel, Maria Elena Bottazzi, Peter Hotez and Susana Mendez
volume 1 | issue 3
May/June 2005Pages: 123 - 128
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The recombinant larval protein Na-ASP-2 from the human helminth parasite Necator americanus is a lead candidate for a human hookworm vaccine. We have characterized the humoral and cellular immune responses elicited by the Na-ASP-2 formulated with the aluminum-based adjuvant Alhydrogel® in the Sprague Dawley rat. We demonstrated that Na-ASP-2 vaccine induced a strong antibody response at all doses tested, as evidenced by high specific IgG1, IgG2a and IgM titers. High IgG antibody titers were maintained up to three months post vaccination and were boosted by an additional vaccine dose. Specific cell proliferation and a Th2 cytokine profile were also observed in peripheral blood of all rats immunized with the formulated vaccine. Host IL-6 levels were also significantly elevated. These data provide evidence that the immune response of the Na-ASP-2 vaccine is robust and durable and therefore suitable for the further clinical development of the Na-ASP-2 vaccine.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.