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Review

Problems associated with reporter assays in RNAi studies

Guihua Sun and John J. Rossi

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RISC, RNA induced silence complex;  Rluc, Renilla luciferase;  Fluc, Firefly luciferase; Ago2, Argonaute2;  RNAi, RNA interfering;  miRNA, microRNA;  siRNA small interfering RNA;  LIF, Leukemia inhibitory factor;  RhoB, RAS homolog B;  Nef, negative factor;  TF, transcription factor;  TGR, transcription gene regulation;  PTGR, post transcription gene regulation;  3’UTR, 3’ untranslated regions;  5’UTR, 5’ untranslated regions;  CDS, coding sequence;  PTBP, polypyrimidine tract binding protein;  NFIB, nuclear factor I/B; DGCR8, DiGeorge Critical Region 8.

Authors

Guihua Sun

Graduate School of Biological Science,Beckman Research Institute of the City of Hope Duarte, CA

John J. Rossi Corresponding author: jrossi@coh.org

Beckam Research Institute of the City of Hope; Duarte, California USA

This is an open-access article

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If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.