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Research Paper

RNA-binding specificity of Y-box protein 1

Jinjiang Dong, Argun Akcakanat, David N. Stivers, Jiexin Zhang, Doyil Kim and Funda Meric-Bernstam

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Y-box protein 1 (YB-1) is a multifunctional DNA/RNA-binding protein that regulates transcription and translation. The specificity of YB-1’s RNA binding and its consequences are unknown.  Because expression and subcellular localization of YB-1 have been reported to be important in breast cancer, we determined the specificity and functional impact of YB-1 mRNA-binding in MCF7 breast cancer cells.  We used YB-1 antibodies to immunoprecipitate YB-1 and microarray profiling to compare YB-1-bound and total poly(A) RNA. We demonstrated that YB-1 mRNA-binding was preferential.  Transcript sequences significantly associated with this binding had high GC content.   Selected YB-1 mRNA-binding targets were confirmed by QRT-PCR. However, downregulation of YB-1 levels by siRNA did not affect their RNA or protein expression.  Thus, YB-1 has RNA-binding specificity; however, YB-1 binding does not necessarily regulate the stability or translation of its mRNA targets.  Further study is needed to determine the functional consequences of selective YB-1 mRNA binding.

Authors

Jinjiang Dong

University of Texas MD Anderson Cancer Center; Houston, Texas USA

Argun Akcakanat

University of Texas MD Anderson Cancer Center; Houston, Texas USA

David N. Stivers

University of Texas MD Anderson Cancer Center; Houston, Texas USA

Jiexin Zhang

University of Texas MD Anderson Cancer Center; Houston, Texas USA

Doyil Kim

University of Texas MD Anderson Cancer Center; Houston, Texas USA

Funda Meric-Bernstam

University of Texas MD Anderson Cancer Center; Houston, Texas USA

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If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.