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Research Paper
In vivo Expression of a Group I Intron HEG from the Antisense Strand of Didymium Ribosomal DNA
Steinar D. Johansen, Anna Vader, Eva Sjøttem and Henrik Nielsen
volume 3 | issue 4
october/november/december 2006Pages: 157 - 162
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Two different isolates of the myxomycete Didymium iridis harbour homing endonuclease genes that are expressed from group I introns inserted into identical sites within the small subunit ribosomal DNA. The homing endonuclease proteins are related in sequence, and their gene structures share similar features such as the presence of small spliceosomal introns and functional polyadenylation sites. However, they are transcribed from opposite strands of the ribosomal DNA and presumable by different RNA polymerases. We have previously described the in vivo expression of the I-DirI homing endonuclease from within the ribosomal RNA precursor. In this paper, we demonstrate the in vivo expression of the I-DirII homing endonuclease from the opposite strand of the Didymium rRNA gene. A comparison of the expression strategies of the two genes demonstrates the feasibility of antisense expression and provides insight into nucleolar gene expression.
Authors
Steinar D. Johansen
University of Tromsø; Tromsø, Norway
Anna Vader
University of Tromsø; Tromsø, Norway
Eva Sjøttem
University of Tromsø; Tromsø, Norway
Henrik Nielsen
University of Tromsø; Tromsø, Norway and University of Copenhagen; Copenhagen, Denmark
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.