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Research Paper
Interaction of Immobilized Human Exportin-t with Calf Liver tRNA
Sheng Li and Mathias Sprinzl
volume 3 | issue 4
october/november/december 2006Pages: 145 - 149
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The binding of tRNA species from calf liver to immobilized exportin-t in the presence of ran•GppNHp was examined by affinity chromatography. We observed different eluting behaviors of individual tRNAs. After separating tRNAs on a two-dimensional polyacrylamide gel, the positions of 7 selected tRNAs were identified by Northern hybridization and their relative affinities to immobilized exportin-t•ran•GppNHp complex were estimated in the order of tRNALeuCAG > tRNASerGCU, tRNALeuCAA, tRNASerUGA > tRNASerAGA, tRNALeuAAG > tRNAArgACG. We propose that exportin-t preferentially binds and exports those tRNAs that are rapidly consumed by the protein synthesis machinery.
Authors
Sheng Li
Laboratorium für Biochemie, Universität Bayreuth; Bayreuth, Germany
Mathias Sprinzl
Laboratorium für Biochemie, Universität Bayreuth; Bayreuth, Germany
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.