Keywords:
Archaea,
RNA catalysis,
RNA secondary structure,
RNase P,
tRNA processing
Abstract:
The RNA component of the RNase P complex is found throughout most branches of the tree of life and is principally responsible for removing the 5′ leader sequence from pre-tRNA transcripts during tRNA maturation. RNase P RNA has a number of universal core features, however variations in sequence and structure found in homologs across the tree of life require multiple Rfam covariance search models to detect accurately. We describe a new Rfam search model to enable efficient detection of the diminutive archaeal Type T RNase P RNAs, which are missed by existing Rfam models. Using the new model, we establish effective score detection thresholds, and detect four new RNase P RNA genes in recently completed genomes from the crenarchaeal family Thermoproteaceae.
Patricia P. Chan,
James W. Brown,
*
Todd M. Lowe
Pages 1155 - 1160
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