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Research Paper
Inhibition of HIV-1 Replication by an Improved Hairpin Ribozyme That Includes an RNA Decoy
Alicia Barroso-delJesus, Elena Puerta-Fernandez, Natalia Tapia, Cristina Romero-López, Francisco Jose Sanchez-Luque, Miguel Angel Martinez and Alfredo Berzal-Herranz
volume 2 | issue 2
april/may/june 2005Pages: 075-079
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An anti-Tat hairpin ribozyme and a TAR RNA decoy were combined in one molecule. The chimeric molecule strongly inhibited HIV-1 replication (measured as changes in p24 levels in viral replication assays). The inhibitory action of the ribodecozyme (85%) was significantly greater than that shown by ribozyme and a non-catalytic variant carrying the functional decoy RNA domain (55% and 35%, respectively). This represents a significant improvement of the inhibitory efficiency of the ribozyme. Suggesting there is an additive inhibitory effect on HIV-1 replication by the catalytic and decoy domains. This strategy could be used to create new inhibitor RNAs with enhanced in vivo performance.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.