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Research Paper
Selection by Phage Display of Peptides Targeting the HIV-1 TAR Element
Gaëlle Kolb and Claudine Boiziau
volume 2 | issue 1
january/february/march 2005Pages: 28-33
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As transcription regulatory element, the HIV-1 TAR RNA element is a promising target to inhibit the viral replication ; indeed, ligands of TAR RNA could prevent the transcription trans-activation process. A phage display in vitro selection was undertaken to select peptidic ligands of TAR RNA. In preliminary experiments, the selection was performed in a magnesium rich buffer (3 mM), but only phages targeted to plastic wells or streptavidin emerged ; in addition, a “super-infectious” phage present in the New England Biolabs library (SVSVGMKPSPRP) selected by others with different targets was cloned, due to a high amplification potential. In contrast, the absence of magnesium or an increasing magnesium concentration (0 to 0.5 mM) led to phage selection with 57 amino acid peptides. KDs of 420-550 nM were measured by filter binding assays ; a significant specificity was obtained when TAR target was compared with unrelated RNA targets. Surprisingly, the binding of selected peptides does not depend on the magnesium concentration.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.