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Research Paper
Inhibition of HIV-1 Replication by siRNA Targeting Conserved Regions of gag/pol
Kevin V. Morris, Chris H. Chung, Werner Witke and David J. Looney
volume 2 | issue 1
january/february/march 2005Pages: 17 - 20
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Short interfering RNAs (siRNAs) targeting HIV-1 gag, vif, tat, rev, and host CD4 and CCR5 have been reported to inhibit HIV replication. However, the sequence divergence of HIV and the concentration dependence of siRNA activity represent significant challenges to RNAi mediated inhibition. To determine the parameters of RNAi in suppression of HIV-1 we screened seven siRNA candidates targeting highly conserved regions of gag/pol, based on target site GC content, for antiviral activity at varying concentrations. Only two of these inhibited CA-p24 production more than 50%, 2064 and 2161. Activity varied with concentration, with 100 nM producing optimal suppression. Requirements for target sequence conservation and activity over a range of concentrations may severely limit the number of siRNA candidates for therapeutic development.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.