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Research Paper
The Translation Elongation Factor eEF1B Plays a Role in the Oxidative Stress Response Pathway
Olubunmi Olarewaju, Pedro A. Ortiz, Wasimul Q. Chowdhury, Ishita Chatterjee and Terri Goss Kinzy
volume 1 | issue 2
july/august 2004Pages: 89 - 94
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The multi-subunit guanine nucleotide exchange factor eEF1B for Saccharomyces cerevisiae Translation Elongation Factor 1A (eEF1A) has catalytic (eEF1B") and non-catalytic (eEF1B() subunits. Deletion of the two non-essential genes encoding eEF1Bg has no dramatic effects on total protein synthesis or translational fidelity. Instead, loss of each gene gives resistance to oxidative stress, and loss of both is additive. The level of stress resistance is similar to overexpression of the Yap1p stress transcription factor, and is dependent on the presence of the YAP1 gene. Cells lacking the catalytic eEF1Ba subunit show even greater resistance to CdSO4, with or without eEF1Bg present. Thus, the loss of guanine nucleotide exchange activity promotes the resistance. As nucleotide exchange is a critical regulator of most G-proteins, these results indicate a new mechanism in the growing list of examples of post-transcriptional responses to cellular stress.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.