Article Addendum

Localization of eIF4A-III in the nucleolus and splicing speckles is an indicator of plant stress

Volume 4, Issue 12   December 2009
Pages 1148 - 1151
http://dx.doi.org/10.4161/psb.4.12.9906
Authors: Olga Koroleva, John W.S. Brown and Peter E. Shaw

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Abstract:
The mechanisms of long-term adaptation to low oxygen environment are quite well studied, but little is known about the sensing of oxygen shortage, the signal transduction and the short-term effects of hypoxia in plant cells. We have found that an RNA helicase eIF4A-III, a putative component of the Exon Junction Complex, rapidly changes its pattern of localisation in the plant nucleus under hypoxic conditions. In normal cell growth conditions GFP- eIF4A-III was mainly nucleoplasmic, but in hypoxia stress conditions it moved to the nucleolus and splicing speckles. This transition occurred within 15-20 min in Arabidopsis culture cells and seedling root cells, but took more than 2 h in tobacco BY-2 culture cells. Inhibition of respiration, transcription or phosphorylation in cells and ethanol treatment had similar effects to hypoxia. The most likely consequence is that a certain mRNA population will remain bound to the eIF4A-III and other mRNA processing proteins, rather than being transported from the nucleus to the cytoplasm, and thus its translation will be suspended.

Article Addendum to:
OA Koroleva, G Calder, AF Pendle, SH Kim, D Lewandowska, CG Simpson, IM Jones, JW Brown, PJ Shaw. Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxia. Plant Cell 2009; 21: 1592-606
PMID: 19435936 DOI: 10.1105/tpc.108.060434

Received: August 25, 2009; Accepted: December 31, 1969

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