Recommend Plant Signaling & Behavior (PS&B) to your librarian for 2008. Download form here.

Sign up for Table of Contents Alerts!

PS&B is the official journal of the Society for Plant Neurobiology. Full membership ($60 annually) and student membership ($30 annually) include online access to the journal. Click here to join.

Email this page

Print this page

Article Addendum

Cell wall remodeling in Arabidopsis stamen abscission zones: Temporal aspects of control inferred from transcriptional profiling

Coralie C. Lashbrook and Suqin Cai

Purchase article for $19

Subscribe to this journal for $79/year

Organ shedding requires cell separation within abscission zones (AZs). Functional genomic AZ studies have been limited by their small size and low incidence. Optimization of laser capture microdissection (LCM) for AZs and other specialized cell types in Arabidopsis allowed recent characterization of the floral stamen AZ transcriptome responding to a developmental shedding cue. Analyses focused on 551 AZ transcripts (AZ₅₅₁) that were regulated at the highest statistical significance (P<=0.0001) over five stages of stamen development spanning pre-pollination to organ shed. Here, we seek a fuller understanding of AZ integrity control by relaxing P value restrictions on statistical significance ten-fold to generate an expanded population of 1461 stamen transcripts (AZ₁₄₆₁). Cell wall remodeling functions in AZ₁₄₆₁ are significantly over-represented relative to all transcripts represented on the whole genome GeneChip. Hierarchical clustering of gene expression data corresponding to cell wall-related transcripts suggests a temporal model for AZ remodeling in Arabidopsis stamens destined to abscise.

Addendum to: Cai S, Lashbrook CC. Stamen abscission zone transcriptome profiling reveals new candidates for abscission control. Enhanced retention of floral organs in transgenic plants overexpressing Arabidopsis zinc finger protein2. Plant Physiol 2008; 146:1305-21.

Authors

Coralie C. Lashbrook

Iowa State University, Department of Genetics, Development and Cell Biology, Ames, Iowa USA

Suqin Cai

Iowa State University, Department of Genetics, Development and Cell Biology, Ames, Iowa USA

Purchase article for $19

Subscribe to this journal for $79/year