Article Addendum

Antisense oligodeoxynucleotide inhibition as a potent diagnostic tool for gene function in plant biology

Volume 3, Issue 5   May 2008
Pages 328 - 330
http://dx.doi.org/10.4161/psb.3.5.5341
Authors: Chuanxin Sun, Haile Ghebramedhin, Anna-Stina Höglund and Christer Jansson

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Abstract:
Antisense oligodeoxynucleotide (ODN) inhibition emerges as an effective means for probing gene function in plant cells. Employing this method we have established the importance of the SUSIBA2 transcription factor for regulation of starch synthesis in barley endosperm, and arrived at a model for the role of the SUSIBAs in sugar signaling and source-sink commutation during cereal endosperm development. In this addendum we provide additional data demonstrating the suitability of the antisense ODN technology in studies on starch branching enzyme activities in barley leaves. We also comment on the mechanism for ODN uptake in plant cells.

Received: November 23, 2007; Accepted: November 28, 2007

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