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Article Addendum
14-3-3 isoforms participate in red light signaling and photoperiodic flowering
Kevin M. Folta, Anna-Lisa Paul, John D. Mayfield and Robert J. Ferl
Volume 3, Issue 5may 2008
Pages: 304 - 306
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Members of the 14-3-3 family of proteins participate in signal transduction by modulating flux through various pathways. Potential subfunctionalization within this family has produced a suite of related proteins with diverse client interactions and discrete localization. The associated study assesses the biological roles of two specific 14-3-3 isoforms, using genetic, biochemical and physiological assays to ascertain potential nodes of interaction. Arabidopsis T-DNA insertion mutants representing the υ and µ isoforms exhibited a short, yet clear delay in flowering time on long days. Tests of hypocotyl growth inhibition under narrow bandwidth light indicated a hyposensitivity to red light, while responses to blue and far-red light were normal. These physiological tests suggest a mechanistic link between 14-3-3 proteins, red light sensing, and the pathways that control photoperiodic flowering. The precise entry point into the pathway was assessed using yeast two hybrid assays targeted against specific proteins active in the circadian oscillator, light transduction and photoperiodic flowering. Yeast two hybrid interaction was observed with CONSTANS (CO), and then confirmed with co-immunoprecipitation. Functional interaction with phyB leading to defects in flowering time and direct interaction with CONSTANS circumstantially places these specific 14-3-3 isoforms into the pathway that regulates the transition between vegetative and floral development.
Authors
Kevin M. Folta
Plant Molecular and Cellular Biology Program and Horticultural Sciences Department; University of Florida; Gainesville, Florida USA
Anna-Lisa Paul
Plant Molecular and Cellular Biology Program and Horticultural Sciences Department; University of Florida; Gainesville, Florida USA
John D. Mayfield
Plant Molecular and Cellular Biology Program and Horticultural Sciences Department; University of Florida; Gainesville, Florida USA
Robert J. Ferl
Plant Molecular and Cellular Biology Program and Horticultural Sciences Department; University of Florida; Gainesville, Florida USA
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
