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Article Addendum
The ascorbate peroxidase regulated by H2O2 and ethylene is involved in cotton fiber cell elongation by modulating ROS homeostasis
Yong-Mei Qin, Chun-Yang Hu and Yu-Xian Zhu
volume 3 | issue 3
march 2008Pages: 194 - 196
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Ascorbate peroxidase (APX) is a reactive oxygen species (ROSs) scavenging enzyme involved in regulation of intracellular ROS levels by reduction of H2O2 to water using ascorbate as an electron donor. In New Phytologist (2007) 175: 462-471, we identified a cotton cytosolic APX1 (GhAPX1) that was significantly accumulated during the fast fiber-cell elongation period, through a proteomics approach. Both the transcript levels of GhAPX1 and the total APX activity were highly induced in response to in vitro applied H2O2 or ethylene. Further analysis showed that ethylene promoted H2O2 production 1 day after it was included in the culture medium, suggesting that H2O2 induced cell elongation processes may be placed downstream of the ethylene signal transduction pathway. In this addendum, quantitative real-time RT-PCR showed that only cytosolic APX1, not other cotton APX genes including a second cytosolic APX2, a glyoxysomal and a stromal APXs, was upregulated during fiber cell elongating. Exogenous H2O2 was found to induce ethylene production if wild-type cotton ovules were cultured for a longer period of time, implying that there was a feedback regulatory mechanism from H2O2 to ethylene biosynthesis in modulating cotton fiber development.
Authors
Yong-Mei Qin
National Laboratory of Protein Engineering and Plant Genetic Engineering; College of Life Sciences; Peking University; Beijing, China
Chun-Yang Hu
National Laboratory of Protein Engineering and Plant Genetic Engineering; College of Life Sciences; Peking University; Beijing, China
Yu-Xian Zhu
National Laboratory of Protein Engineering and Plant Genetic Engineering; College of Life Sciences; Peking University; Beijing, China