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Research Paper

Co-Localization of ß-1,3-Glucanases and Callose During Somatic Embryogenesis in Cichorium

Valérie Grimault, Stéphane Helleboid, Jacques Vasseur and Jean-Louis Hilbert

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During direct somatic embryogenesis in leaves of Cichorium hybrid clone '474', 38 kDa ß-1,3-glucanases are accumulated in the culture medium of the embryogenic hybrid to a higher level when compared with a non-embryogenic cultivar. In the same time, embryogenic cells were surrounded by a cell wall that was characterized by the presence of callose. This callosic deposition disappeared as embryos grew. Callose consisted of ß-1,3-glucan linkages and so represented a possible substrate for ß-1,3-glucanases. Using immunolocalization experiments, we demonstrated that from the three types of callose deposits observed during the culturing of Cichorium leaf explants, only the callose present in the walls surrounding reactivated cells seemed specifically related to somatic embryogenesis. Moreover, callose and the 38-kDa ß-1,3-glucanases were co-localized dispersed throughout the thick and swelled walls of reactivated cells and embryo cell walls. This suggests that callose and ß-1,3-glucanases are implicated in the process of somatic embryogenesis since they were always detected in or quite near embryogenic and embryo cell. This also suggested that ß-1,3-glucanases could be involved in the degradation of this callose.

Authors

Valérie Grimault

Université des Sciences et Technologies de Lille, Villeneuve, France

Stéphane Helleboid

Université des Sciences et Technologies de Lille, Villeneuve, France

Jacques Vasseur

Université des Sciences et Technologies de Lille, Villeneuve, France

Jean-Louis Hilbert

Universit 0064es Sciences et Technologies de Lille, Villeneuve, France

Purchase article for $19

Subscribe to this journal for $79/year