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Research Paper
Glutathione-S-transferase is Detected During Somatic Embryogenesis in Chicory
Rachel Galland, Anne-Sophie Blervacq, Christelle Blassiau, Benoît Smagghe, Jean-Pierre Decottignies and Jean-Louis Hilbert
volume 2 | issue 5
september/octoberPages: 343 - 348
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Glutathione S-tranferases (GSTs) are a heterogeneous family of proteins, which perform diverse pivotal catalytic and non-enzymatic functions during plant development and in plant stress responses. Previous studies have shown that a GST activity (EC 2.5.1.18) is closely linked with the precocious phases of somatic embryogenesis in leaf tissues of an interspecific chicory hybrid (Cichorium intybus L. var. sativa x C. endivia L.var. latifolia). In order to learn more about the involvement of this enzyme in this process, in situ-hybridization as well as immunolocalization were performed in parallel. GST-mRNAs and proteins were co-localized in small veins, particularly in young protoxylem cell walls. During cell reactivation, the in situ and protein signals became less intense and were associated with chloroplasts. The GST-mRNAs and corresponding proteins were not always co-localized in the same tissues. While high amounts of transcripts could be detected in multicellular embryos, the proteins were not well labeled. Our results indicated that GSTs belong to a complex anti-oxidant mechanism within the cell, and also at the cell wall level. GSTs presence in reactivated cell and multicellular embryos is discussed in relation to redox cell status.
Authors
Rachel Galland
Université des Sciences et Technologies de Lille, Stress Abiotiques et Différenciation des Végétaux Cultivés, Villeneuve, France
Anne-Sophie Blervacq
Université des Sciences et Technologies de Lille, Stress Abiotiques et Différenciation des Végétaux Cultivés, Villeneuve, France
Christelle Blassiau
Université des Sciences et Technologies de Lille, Stress Abiotiques et Différenciation des Végétaux Cultivés, Villeneuve, France
Benoît Smagghe
Université des Sciences et Technologies de Lille, Stress Abiotiques et Différenciation des Végétaux Cultivés, Villeneuve, France
Jean-Pierre Decottignies
Université des Sciences et Technologies de Lille, Villeneuve, France
Jean-Louis Hilbert
Universit 0064es Sciences et Technologies de Lille, Villeneuve, France
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.