Email this page

Print this page

Article Addendum

Arabidopsis Protein Microarrays for the High-Throughput Identification of Protein-Protein Interactions

Sorina C. Popescu, Michael Snyder and Savithramma P. Dinesh-Kumar

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.

Protein microarray technology has emerged as a powerful new approach for the study of thousands of proteins simultaneously. Protein microarrays have been used for a wide variety of applications for the human and yeast systems. In a recent study, we demonstrated that Arabidopsis functional protein microarrays can be generated and employed to characterize the function of plant proteins. The arrayed proteins were produced using an optimized large-scale plant-based expression system. In a proof-of concept study, 173 known and novel potential substrates of calmodulin (CaM) and calmodulin-like proteins (CML) were identified in an unbiased and high-throughput manner. The information documented here on novel potential CaM targets provides new testable hypotheses in the area of CaM/Ca2+-regulated processes and represents a resource of functional information for the scientific community.

Authors

Sorina C. Popescu

Yale University, New Haven, CT

Michael Snyder

Yale University, New Haven, CT

Savithramma P. Dinesh-Kumar

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.