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Article Addendum
A Proteomics Approach Highlights a Myriad of Transporters in the Arabidopsis thaliana Vacuolar Membrane
Michel Jaquinod, Florent Villiers, Sylvie Kieffer-Jaquinod, Véronique Hugouvieux, Christophe Bruley, Jerome Garin and Jacques Bourguignon
volume 2 | issue 5
september/octoberPages: 412 - 414
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To better understand plant vacuolar functions and identify new transporters present on the tonoplast, a proteomic work was initiated on Arabidopsis thaliana. A procedure was developed to prepare highly purified vacuoles from protoplasts isolated from Arabidopsis cell cultures, and a proteomics approach was designed to identify the protein components present in both the membrane and soluble fractions of the vacuoles. This procedure allowed the identification of 650 proteins, 2/3 of which copurify with the hydrophobic membrane fraction and 1/3 with the soluble fraction. With regard to function, only 20% of the proteins identified were previously known to be associated with vacuolar activities.
Authors
Michel Jaquinod
Institut de Recherches en Technologies et Sciences pour le Vivant, Grenoble France
Florent Villiers
Laboratoire de Physiologie Cellulaire Végétale, Grenoble France
Sylvie Kieffer-Jaquinod
Institut de Recherches en Technologies et Sciences pour le Vivant, Grenoble France
Véronique Hugouvieux
Laboratoire de Physiologie Cellulaire Végétale, Grenoble France
Christophe Bruley
Institut de Recherches en Technologies et Sciences pour le Vivant, Grenoble France
Jerome Garin
Institut de Recherches en Technologies et Sciences pour le Vivant, Grenoble France
Jacques Bourguignon
Laboratoire de Physiologie Cellulaire Végétale, Grenoble France
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.