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Research Papers
Altered Expression of PERK Receptor Kinases in Arabidopsis Leads to Changes in Growth and Floral Organ Formation
Yosr Haffani, Nancy Silva-Gagliardi, Sarah Sewter, May Grace Aldea, Zhiying Zhao, Alina Nakhamchik, Robin Cameron and Daphne Goring
volume 1 | issue 5
september/october 2006Pages: 251 - 260
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The Proline-rich, Extensin-like Receptor Kinase (PERK) family is characterized by a putative extracellular domain related to cell wall proteins, followed by a transmembrane domain and kinase domain. The original member, PERK1, was isolated from Brassica napus (BnPERK1), and 15 PERK1-related members were subsequently identified in the Arabidopsis thaliana genome. Ectopic expression and antisense suppression studies were performed using the BnPERK1 cDNA under the control of the 35S CaMV constitutive promoter and introduced into Arabidopsis. In the case of antisense suppression, the BnPERK1 cDNA shared sufficient sequence similarity to suppress several members of the AtPERK family. In both sets of transgenic Arabidopsis, several heritable changes in growth and development were observed. Antisense BnPERK1 transgenic Arabidopsis showed various growth defects including loss of apical dominance, increased secondary branching, and floral organ defects. In contrast, Arabidopsis plants ectopically expressing BnPERK1 displayed a prolonged lifespan with increased lateral shoot production and seed set. Along with these phenotypic changes, aberrant deposits of callose and cellulose were also observed, suggestive of cell wall changes as a consequence of altered PERK expression.
Authors
Yosr Haffani
Samuel Lunenfeld Research Institute, Mount Sinai Hospital
Nancy Silva-Gagliardi
The Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children
Sarah Sewter
University of Toronto
May Grace Aldea
University of Toronto
Zhiying Zhao
Ceres Inc
Alina Nakhamchik
Sunnybrook & Women’s College Health Sciences Center
Robin Cameron
McMaster University
Daphne Goring
University of Toronto
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.