Downloads and Tools

•  Article PDF
  2.23 MB PDF document

This is an open access article.
Print this page Email this page

Recipient's Email:

---

Article Citation

RIS
MARC (XML)
FULL CITATION (TXT)

---

Share on Facebook

Authors: Ed Etxeberria, Pedro Gonzalez, Edurne Baroja-Fernández and Javier Pozueta Romero

Ed Etxeberria

Corresponding author: eje@crec.ifas.ufl.edu
University of Florida; Institute of Food and Agricultural Sciences; Citrus Research and Education Center; Department of Horticultural Sciences; Lake Alfred, FL USA

Pedro Gonzalez

University of Florida; Institute of Food and Agricultural Sciences; Citrus Research and Education Center; Department of Horticultural Sciences; Lake Alfred, FL USA

Edurne Baroja-Fernández

Agrobioteknologia Instituta; Consejo Superior de Investigaciones Cientificas and Nafarroako Unibertsitate Publikoa; Mutiloako etorbidea zembaki gabe; Mutiloabeti, Nafarroa, Spain

Javier Pozueta Romero

Agrobioteknologia Instituta; Consejo Superior de Investigaciones Cientificas and Nafarroako Unibertsitate Publikoa; Mutiloako etorbidea zembaki gabe; Mutiloabeti, Nafarroa, Spain

Abstract:
Fluid phase endocytic uptake of external solutes in plant cells was further substantiated using artificial polystyrene nano-spheres (40 nm) and CdSe/ZnS quantum dots (20 nm). Both types of artificial nano particles were taken up by sycamore cultured cells. However, whereas polystyrene nano-spheres were delivered to the central vacuole, CdSe/ZnS nano-dots were sequestered into cytoplasmic vesicular structures. Using dextran Texas Red (m.w. 3,000; d-TR) as additional marker, confocal micrographs confirmed the distinct topographic distribution of CdSe/ZnS quantum dots within the cell. Initially, d-TR and CdSe/ZnS quantum dots co-localized within cytoplasmic vesicles. After 18 h incubation, d-TR was distinctly localized in the vacuole whereas CdSe/ZnS quantum dots remained sequestered in cytoplasmic membranous compartments. The data provide a first evidence for the rapid distribution of solutes taken up by endocytosis to distinct intracellular compartments.

Preview: