Illumination of Arabidopsis roots induces immediate burst of ROS production
Volume 6, Issue 10
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Pages 1460 - 1464http://dx.doi.org/10.4161/psb.6.10.18165
Authors: Ken Yokawa , Tomoko Kagenishi , Tomonori Kawano, Stefano Mancuso and František Baluška View affiliations
Arabidopsis roots are routinely exposed to light both during their cultivation within transparent Petri dishes and during their confocal microscopy analysis. Here we report that illumination of roots which naturally grow in darkness, even for a few seconds, induces an immediate and strong burst of reactive oxygen species (ROS). Plant scientists studying roots should pay great attention to the environment of living roots, and keep them in darkness as long as possible. Results obtained using illuminated roots during in vivo microscopic analysis should also be interpreted with great caution.
Received: July 14, 2011; Accepted: September 20, 2011