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Short Communication

Amyloid-like properties of Saccharomyces cerevisiae cell wall glucantransferase Bgl2p: prediction and experimental evidences

Tatyana S. Kalebina, Tatyana A. Plotnikova, Anton A. Gorkovskii, Irina O. Selyakh, Oxana V. Galzitskaya, Evgeniy E. Bezsonov, Gerd Gellissen and Igor S. Kulaev

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Glucantransferase Bgl2p is a major conserved cell wall constituent described for a wide range of yeast species. In the baker’s yeast Saccharomyces cerevisiae it is the only non-covalently bound cell wall protein that cannot be released from cell walls by sequential SDS and trypsin treatment. It contains 7 amyloidogenic determinants. Circular dichroism analysis and fluorescence spectroscopy with thioflavin T indicate the presence of β-sheet structures in Bgl2p isolates. Bgl2p forms fibrils, a process that is enforced in the presence of other cell wall components. Thus the data obtained is the first evidence for amyloid-like properties of yeast cell wall protein – glucantransferase Bgl2p.

Authors

Tatyana S. Kalebina

Lomonosov Moscow State University

Tatyana A. Plotnikova

Lomonosov Moscow State University

Anton A. Gorkovskii

Lomonosov Moscow State University; Russian Academy of Sciences

Irina O. Selyakh

Lomonosov Moscow State University

Oxana V. Galzitskaya

Russian Academy of Sciences

Evgeniy E. Bezsonov

Lomonosov Moscow State University

Gerd Gellissen

Pharmedartis GmbH

Igor S. Kulaev

Lomonosov Moscow State University; Russian Academy of Sciences

Purchase article for $19

Subscribe to this journal for $59/year