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Review: Imaging

Live optical projection tomography

Jean-François Colas and James Sharpe
Volume 5, Issue 4
October/November/December 2009
Pages 211 - 216
DOI: 10.4161/org.5.4.10426

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Optical projection tomography (OPT) is a technology ideally suited for imaging embryonic organs. We emphasize here recent successes in translating this potential into the field of live imaging. Live OPT (also known as 4D OPT, or time-lapse OPT) is already in position to accumulate good quantitative data on the developmental dynamics of organogenesis, a prerequisite for building realistic computer models and tackling new biological problems. Yet, live OPT is being further developed by merging state-of-the-art mouse embryo culture with the OPT system. We discuss the technological challenges that this entails and the prospects for expansion of this molecular imaging technique into a wider range of applications.


Authors

Jean-François Colas Corresponding author: jean-francois.colas@crg.es
Centre for Genomic Regulation; Barcelona, Spain
James Sharpe
Centre for Genomic Regulation; Barcelona, Spain

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