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Research Paper

The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells

Mugdha V. Joglekar, Deepak Patil, Vinay M. Joglekar, GV Rao, D Nageshwar Reddy, Sasikala Mitnala, Yogesh Shouche and Anandwardhan A. Hardikar

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Epithelial-to-mesenchymal transition is a phenomenon necessary for embryonic development and also seen during certain pathological conditions.  We show here for the first time that reduction in miR-30 family microRNAs, is responsible for mesenchymal transition of primary cultures of human pancreatic epithelial cells.  We found that miR-30 family microRNAs target mesenchymal gene transcripts and maintain them in a translationally inactive state.  Forced depletion using miR-30 family specific anti-miRs leads to mesenchymal transition while ectopic overexpression maintains the epithelial phenotype.  We also show that miR-30 family microRNAs increase in abundance during differentiation of pancreatic islet-derived mesenchymal cells into hormone-producing islet-like cell aggregates.  Our studies in human adult diseased pancreas also demonstrate that miR-30 family microRNAs are expressed at lower abundance in fibrotic lesions during pancreatitis.  Together, our data confirm that miR-30 family microRNAs form a part of the regulatory signaling events involved in cellular response of pancreatic epithelial cells during mesenchymal transition.

Authors

Mugdha V. Joglekar

Stem Cells and Diabetes Section, National Center for Cell Science, Ganeshkhind Road, Pune India

Deepak Patil

DNA Sequencing Facility, National Center for Cell Science, Ganeshkhind Road, Pune MH, India

Vinay M. Joglekar

Shree Seva Medical Foundation, Shirwal, Satara, MH, India

GV Rao

Asian Institute of Gastroenterology, 63661, Somajiguda, Hyderabad AP, India

D Nageshwar Reddy

Asian Institute of Gastroenterology, 63661, Somajiguda, Hyderabad AP, India

Sasikala Mitnala

Institute of Basic Sciences, Asian Healthcare Foundation, Somajiguda, Hyderabad, India

Yogesh Shouche

DNA Sequencing Facility, National Center for Cell Science, Ganeshkhind Road, Pune MH, India

Anandwardhan A. Hardikar Corresponding author: anand@isletbiology.com

Stem Cells and Diabetes Section, National Center for Cell Science, Ganeshkhind Road, Pune India

This is an open-access article

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.