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Authors: Claudio Vinegoni, Sungon Lee, Rostic Gorbatov and Ralph Weissleder

Claudio Vinegoni

Corresponding author: cvinegoni@mgh.harvard.edu
Center for System Biology; Massachusetts General Hospital and Harvard Medical School; Boston MA, USA; Center for Molecular Imaging Research; Massachusetts General Hospital and Harvard Medical School; Charlestown, MA USA
These authors contributed equally to this work.

Sungon Lee

Center for System Biology; Massachusetts General Hospital and Harvard Medical School; Boston MA, USA; Interaction and Robotics Research Center; Korea Institute of Science and Technology; Seoul, Korea
These authors contributed equally to this work.

Rostic Gorbatov

Center for System Biology; Massachusetts General Hospital and Harvard Medical School; Boston MA, USA

Ralph Weissleder

Center for System Biology; Massachusetts General Hospital and Harvard Medical School; Boston MA, USA; Center for Molecular Imaging Research; Massachusetts General Hospital and Harvard Medical School; Charlestown, MA USA

Abstract:
Motion artifacts continue to present a major challenge to single cell imaging in cardiothoracic organs such as the beating heart, blood vessels or lung. In this study, we present a new water-immersion suctioning stabilizer that enables minimally invasive intravital fluorescence microscopy using water-based stick objectives. The stabilizer works by reducing major motion excursions and can be used in conjunction with both prospective or retrospective gating approaches. We show that the new approach offers cellular resolution in the beating murine heart without perturbing normal physiology. In addition, because this technique allows multiple areas to be easily probed, it offers the opportunity for wide area coverage at high resolution.

Received: September 16, 2012; Accepted: November 27, 2012

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