Motion compensation using a suctioning stabilizer for intravital microscopy
Volume 1, Issue 2
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Pages 115 - 121http://dx.doi.org/10.4161/intv.23017
: cardiac imaging, in vivo imaging, intravital microscopy, motion compensation, optical microscopy
Authors: Claudio Vinegoni, Sungon Lee, Rostic Gorbatov and Ralph Weissleder View affiliations
Motion artifacts continue to present a major challenge to single cell imaging in cardiothoracic organs such as the beating heart, blood vessels or lung. In this study, we present a new water-immersion suctioning stabilizer that enables minimally invasive intravital fluorescence microscopy using water-based stick objectives. The stabilizer works by reducing major motion excursions and can be used in conjunction with both prospective or retrospective gating approaches. We show that the new approach offers cellular resolution in the beating murine heart without perturbing normal physiology. In addition, because this technique allows multiple areas to be easily probed, it offers the opportunity for wide area coverage at high resolution.
Received: September 16, 2012; Accepted: November 27, 2012