Methods Article

DISC: A guide to in vivo cytometry

Volume 1, Issue 1   July/August/September 2012
Pages 27 - 31
http://dx.doi.org/10.4161/intv.21896
Keywords: DISC, cell tracking, data analysis, flow cytometry, immunoimaging
Authors: Hélène Moreau and Philippe Bousso

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Abstract:
Dynamic in situ cytometry (DISC) is a new approach that combines advantages of both intravital imaging and flow cytometry analysis. The DISC methodology provides the opportunity to link cell behavior and phenotype in vivo and hence to perform cytometry in vivo and in real-time. In addition it enables you to readily display and analyze multiparametric imaging data. Through concrete examples, we present a guide to perform DISC experiments with the help of the custom designed DISCit software.

Received: July 3, 2012; Accepted: August 20, 2012

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