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Expression Pattern Report

Imp (IGF-II mRNA-Binding Protein) Is Expressed During Spermatogenesis in Drosophila melanogaster

James Fabrizio, Christina A. Hickey, Cecylia Stabrawa, Vadim Meytes, Jessica A. Hunter, Caitlin Talbert and Nadine Regis

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Drosophila spermatogenesis results in the production of sixty four ~2mm spermatozoa from an individual founder cell. Little is known, however, about the elongation of spermatids to such an extraordinary length. In a partial screen of a GFP-tagged protein trap collection, four insertions were uncovered that exhibit expression toward the tail ends of spermatid cysts and within the apical tip of the testis, suggesting that these protein traps may represent genes involved in spermatid elongation and pre-meiotic spermatogenesis, respectively. Inverse PCR followed by cycle sequencing and BLAST revealed that all four protein traps represent insertions within Imp (IGFII-mRNA binding protein), a known translational regulator. Testis enhancer trap analysis also reveals Imp expression in the cells of the apical tip, suggesting transcription of Imp prior to the primary spermatocyte stage. Taken together, these results suggest a role for Imp in the male germline during both spermatid elongation and pre-meiotic spermatogenesis.

Authors

James Fabrizio

College of Mount Saint Vincent

Christina A. Hickey

Manhattan College

Cecylia Stabrawa

Manhattan College

Vadim Meytes

Manhattan College

Jessica A. Hunter

Manhattan College

Caitlin Talbert

Manhattan College

Nadine Regis

Manhattan College

Purchase article for $19

Subscribe to this journal for $79/year