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Methods and Technical Advances
A Green Fluorescent Protein-Based Reporter for Protein Nuclear Import Studies in Drosophila Cells
Wing Man Chan, Pang Chui Shaw and Ho Yin Chan
volume 1 | issue 6
November/December 2007Pages: 340 - 342
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Green fluorescent protein-based reporters are commonly used to investigate protein nucleocytoplasmic transport. In this study we developed a novel reporter GFP2-GST which consists of 2 copies of GFP and 1 copy of GST, and tested it in two commonly used Drosophila cell lines. The size of the GFP2-GST reporter exceeds the passive diffusion limit across the nuclear pore complexes. It shows an exclusive cytoplasmic localization and displays a restrictive nuclear localization when a nuclear localization signal is appended. This reporter will largely facilitate the characterization and identification of NLS sequences in the fly proteome.
Authors
Wing Man Chan
The Chinese University of Hong Kong
Pang Chui Shaw
The Chinese University of Hong Kong
Ho Yin Chan
The Chinese University of Hong Kong