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Allele Report

The Generation and Analysis of Deficiencies Within a Small Genomic rRegion on the X Chromosome of Drosophila melanogaster Containing Two Genes, enhancer of rudimentary and CG15352

Margarita Roykhman, Suzanne Ibach, Deloris Harding, Brian Wolff, Melissa Vowels, Mark E. Gelsthorpe, Edward J. Wojcik, Nicholas P. Rizzo and Stuart I. Tsubota

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The enhancer of rudimentary gene, e(r), encodes a 104-amino-acid, highly conserved transcription cofactor. Hypomorphic mutations of e(r) show an enhancement of a hypomorphic rudimentary mutant wing phenotype. These mutants in a wild-type background are viable, fertile, and morphologically wild-type. Since the only mutant alleles were hypomorphic, it was important to isolate null mutations to determine if any other phenotypes might be associated with a loss-of-function of e(r). We utilized a marked P element, P{SUPor-P, y+}, located 895 bp upstream of the start of transcription of e(r) to generate nineteen deficiencies in the region. Deficiencies of e(r) enhance the mutant wing phenotype of a hypomorphic rudimentary allele, rhd1. In a wild-type background, the deficiencies of e(r), unlike the hypomorphic alleles, have a low viability and females have low fertility. The expression of e(r) in the nurse cells of the ovary is consistent with the low fertility, and suggests an ovarian function for e(r). Deficiencies of CG15352, the gene directly upstream of e(r), are not associated with any obvious mutant phenotypes and present the possibility that it encodes a non-vital or redundant function.

Authors

Margarita Roykhman

St. Louis University

Suzanne Ibach

St. Louis University

Deloris Harding

St. Louis University

Brian Wolff

St. Louis University

Melissa Vowels

St. Louis University

Mark E. Gelsthorpe

St. Louis University

Edward J. Wojcik

LSU Health Sciences Center

Nicholas P. Rizzo

SUNY Brockport

Stuart I. Tsubota

SUNY Brockport

This is an open-access article

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