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Research Papers
A Drosophila Overexpression Screen for Modifiers of Rho Signalling in Cytokinesis
Stephen L. Gregory, Tetyana Shandala, Louise O'Keefe, Lynn Jones, Michael J. Murray and Robert Saint
volume 1 | issue 1
January/February 2007We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
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To identify genes that modulate Rho signalling during cytokinesis we tested the effect of overexpressing a set of 2190 genes on an eye phenotype caused by defective Rho activation. The resulting 112 modifier loci fell into three main classes: cell cycle genes, signalling effectors and metabolic enzymes. We developed a further series of genetic tests to refine the interactors into those most likely to modify Rho signalling during cytokinesis. In addition to a number of genes previously implicated in the Rho pathway during cytokinesis, we identified four novel primary candidates: cdc14, Pitslre, PDK1 and thread/diap1. cdc14 orthologs have, however, been implicated in cytokinesis in other organisms, as have molecules related to Thread/Diap1. The identification of several modifiers that are genetically redundant paralogs highlights the ability of overexpression screens to identify genes that are refractory to traditional loss-of-function approaches. Overexpression screens and sensitized phenotypes, therefore, may help identify the many factors that are expected to be involved in cytokinesis but have not been discovered by previous genetic screens.
Authors
Stephen L. Gregory
University of Adelaide
Tetyana Shandala
University of Adelaide
Louise O'Keefe
University of Adelaide
Lynn Jones
Australian National University
Michael J. Murray
Australian National University
Robert Saint
Australian National University
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.