Methods and Technical Advances

A novel genetic tool for clonal analysis of fourth chromosome mutations

Volume 6, Issue 1   January/February/March 2012
Pages 49 - 56
http://dx.doi.org/10.4161/fly.18415
Keywords: confocal microscopy, fourth chromosome, GAL4/GAL80, site-directed recombination, somatic clones
Authors: Rui Sousa-Neves and Joseph M. Schinaman

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Abstract:

The fourth chromosome of Drosophila remains one of the most intractable regions of the fly genome to genetic analysis. The main difficulty posed to the genetic analyses of mutations on this chromosome arises from the fact that it does not undergo meiotic recombination, which makes recombination mapping impossible, and also prevents clonal analysis of mutations, a technique which relies on recombination to introduce the prerequisite recessive markers and FLP-recombinase recognition targets (FRT). Here we introduce a method that overcomes these limitations and allows for the generation of single Minute haplo-4 clones of any fourth chromosome mutant gene in tissues of developing and adult flies.

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