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Research Paper
Using LUMA: a Luminometric Based Assay for global DNA-Methylation
Mohsen Karimi, Sofia Johansson and Tomas J. Ekström
volume 1 | issue 1
january/february/march 2006Pages: 45 - 48
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Changes in genomic DNA methylation are important events in normal and pathological
cellular processes, contributing both to normal development and differentiation as well as to
cancer and other diseases. We describe here a method to analyze global genomic DNA
methylation, using a luminometric technology to quantitate methylation sensitive restriction
digestions. The method is called LUminometric Methylation Assay (LUMA), and is based on
a polymerase extension assay using the the Pyrosequencing™ platform. The method is
quantitative, highly reproducible and uses an internal control for DNA input. No modification
of genomic DNA is needed and the total running time is only six hours. The method is
suitable for analyzing clinical material, as well as determining dynamic changes in global
methylation/demethylation events.
This report describes the method in detail and gives an example of its application in
epigenetic research.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.