Standardization and quality controls for the methylated DNA immunoprecipitation technique
Volume 7, Issue 6
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Pages 615 - 625http://dx.doi.org/10.4161/epi.20028
: CpG methylation, MeDIP normalization, MeDIP quality controls, Methylated DNA Immunoprecipitation, Whole genome amplification
Authors: Sofia Lisanti, Thomas von Zglinicki and John C. Mathers View affiliations
MeDIP (Methylated DNA Immunoprecipitation) is a relatively recent technique aimed to enrich the methylated fraction of DNA with an antibody directed against 5-methyl-cytosine. MeDIP processed samples are suitable for investigation of the methylation status of specific genomic loci and for performing genome-wide screening when hybridized to DNA methylation microarrays or analyzed by deep sequencing. Here, we describe a standardization protocol and quality controls to assess the specificity, reproducibility and efficiency of the MeDIP procedure. These may have utility when comparing results between samples and experiments within laboratories and between laboratories.
Received: November 22, 2011; Accepted: March 14, 2012