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Methods and Technical Advances

Accurate sodium bisulfite sequencing in plants

Ian R. Henderson, Simon R. Chan, Xiaofeng Cao, Lianna Johnson and Steven E. Jacobsen

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DNA cytosine methylation is a conserved epigenetic modification frequently correlating with transcriptional silencing in a wide variety of eukaryotic organisms. Sodium bisulfite treatment of DNA converts unmethylated cytosine to uracil, while 5-methylated cytosine is protected. We describe techniques that ensure reliable sequencing data following sodium bisulfite conversion and to avoid common pitfalls such as amplification of unconverted DNA and inclusion of sibling clones.

Authors

Ian R. Henderson

University of Cambridge

Simon R. Chan

University of California, Davis

Xiaofeng Cao

Chinese Academy of Sciences

Lianna Johnson

University of California, Los Angeles

Steven E. Jacobsen Corresponding author: jacobsen@ucla.edu

University of California, Los Angeles

This is an open-access article

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.