• Longitudinal epigenetic drift in mice perinatally exposed to lead
  • The clustering of CpG islands may constitute an important determinant of the 3D organization of interphase chromosomes
  • Epigenome-wide association study reveals longitudinally stable DNA methylation differences in CD4+ T cells from children with IgE-mediated food allergy

1 May 2014

Longitudinal epigenetic drift in mice perinatally exposed to lead

Christopher Faulk, Kevin Liu, Amanda Barks, Jaclyn M Goodrich and Dana C Dolinoy

An understanding of the natural change in DNA methylation over time, defined as “epigenetic drift,” will inform the study of environmental effects on the epigenome. This study investigates epigenetic drift in isogenic mice exposed perinatally to lead (Pb) acetate at four concentrations, 0 ppm (control), 2.1 ppm (low), 16 ppm (medium), and 32 ppm (high) prior to conception through weaning, then followed until 10 months of age. Absolute values of DNA methylation in a transposon-associated metastable locus, Cdk5-activator binding protein (CabpIAP), and three imprinted loci (Igf2, Igf2r, and H19) were obtained from tail tissue in paired samples. DNA methylation levels in the controls increased over time at the imprinted Igf2 and Igf2r loci (both P = 0.0001), but not at the imprinted H19 locus or the CabpIAP metastable epiallele. Pb exposure was associated with accelerated DNA hypermethylation in CabpIAP (P = 0.0209) and moderated hypermethylation in Igf2r (P = 0.0447), and with marginally accelerated hypermethylation at H19 (P = 0.0847). In summary, the presence and magnitude of epigenetic drift was locus-dependent, and enhancement of drift was mediated by perinatal Pb exposure, in some, but not all, loci.


15 Apr 2014

The clustering of CpG islands may constitute an important determinant of the 3D organization of interphase chromosomes

Ekaterina S Gushchanskaya, Artem V Artemov, Sergey V Ulyanov, Maria D Logacheva, Aleksey A Penin, Elena S Kotova, Sergey B Akopov, Lev G Nikolaev, Olga V Iarovaia, Eugene D Sverdlov, Alexey A Gavrilov and Sergey V Razin


24 Apr 2014

Epigenome-wide association study reveals longitudinally stable DNA methylation differences in CD4+ T cells from children with IgE-mediated food allergy

David Martino, Jihoon E Joo, Alexandra Sexton-Oates, Thanh Dang, Katrina Allen, Richard Saffery and Susan Prescott

Current Issue

Epigenetics

July 2014

Volume 9, Issue 7

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About the featured image
Unsupervised hierarchical clustering of Pyrosequencing data for genes that were found methylated in SI-NETs. For more information see Fotouhi et al.

Recent Articles

Editor-in-Chief

Manel Esteller
Cancer Epigenetics and
Biology Program
Barcelona, Spain

Executive Editor

Barbara P Rattner
Landes Bioscience