Article Addendum

Cadaveric bone marrow as potential source of hematopoietic stem cells for transplantation

Volume 2, Issue 3   July/August/September 2011
Pages 86 - 87
http://dx.doi.org/10.4161/chim.17917
Authors: Jana Michalova, Filipp Savvulidi, Ludek Sefc, Katarina Forgacova and Emanuel Necas

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Abstract:
Every year, bone marrow transplantation saves many lives worldwide. Unfortunately, a suitable donor is not always available. Since organs are routinely harvested from cadaveric organ donors, we decided to assess such a possibility for bone marrow. We analyzed the functional properties and phenotypic markers of murine hematopoietic stem and progenitor cells (HSPC) from cadaveric bone marrow and during storage in vitro in a suspension. It was demonstrated that HSPC exposed to a warm or cold ischemia in intact femur did not lose their phenotype and maintained their repopulating ability for 2 to 12 hours depending on the temperature. Additionally, fresh bone marrow remained fully viable in cell suspension for 2 days or 4 days at 37°C or 4°C, respectively. Based on these findings, cadaveric bone marrow could be considered as an alternative source of hematopoietic stem cells for transplantation.

Article Addendum to:
J Michalova, F Savvulidi, L Sefc, K Faltusova, K Forgacova, E Necas. Hematopoietic stem cells survive circulation arrest and reconstitute hematopoiesis in myeloablated mice. Biol Blood Marrow Transplant 2011; 17: 1273-1281
PMID: 21767513 DOI: 10.1016/j.bbmt.2011.07.007

Received: August 22, 2011; Accepted: August 30, 2011

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