Letter to the Editor

CD 30 is a marker of undifferentiated human embryonic stem cells rather than a biomarker of transformed hESCs

Volume 7, Issue 22   November 15, 2008
Pages 3610 - 3612
http://dx.doi.org/10.4161/cc.7.22.6981
Authors: Maria A. Lagarkova, Pavel Y. Volchkov, Elena S. Philonenko, Kurt Pfannkuche, Maria A. Prokhorovich, Tatyana Zabotina, Juergen Hescheler and Sergei L. Kiselev

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Abstract:
Recently it has been demonstrated that CD30 expression was rather specific for transformed than for normal human ES cells and therefore CD30 maybe suggested as a potential marker for human ES cells bearing chromosomal abnormalities. Using immunohistochemistry and RT-PCR analysis we examined СD30 expression in 10 hESCs lines with normal and abberant karyotypes. All hESC lines expressed CD30 antigen and RNA in undifferentiated state whether cell line beared chromosomal abnormalities or not. In contrast to previous notions our data demonstrate that CD30 could be considered as marker of undifferentiated hESCs without respect to karyotype changes.

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